DNA

Part:BBa_K3289012:Design

Designed by: Tatiana Houhou   Group: iGEM19_NYU_Abu_Dhabi   (2019-10-21)


HbcAg for the Detection of Hepatitis B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
    Illegal XbaI site found at 247
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BglII site found at 529
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
    Illegal XbaI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal XbaI site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI.rc site found at 251


Design Notes

When we ordered the gblock from IDT, we requested that it is 5' end phosphorylated to facilitate the blunt end ligation into the pJET backbone vector which is then transformed into E coli. for DNA cloning.


Source

HBV, Hepatitis B Virus

References

https://www.cdc.gov/hepatitis/hbv/index.htm

Kim, Bo Hyun, and W. Ray Kim. "Epidemiology of hepatitis B virus infection in the United States." Clinical liver disease 12.1 (2018): 1.

Kim, W Ray. “Epidemiology of hepatitis B in the United States.” Hepatology (Baltimore, Md.) vol. 49,5 Suppl (2009): S28-34. doi:10.1002/hep.22975

https://www.ncbi.nlm.nih.gov/nuccore/NC_003977.2?report=fasta&from=1816&to=2454