![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K3289012:Design
Designed by: Tatiana Houhou Group: iGEM19_NYU_Abu_Dhabi (2019-10-21)
HbcAg for the Detection of Hepatitis B
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 96
Illegal XbaI site found at 247 - 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 90
Illegal BglII site found at 529 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 96
Illegal XbaI site found at 247 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 96
Illegal XbaI site found at 247 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 506
Illegal BsaI.rc site found at 251
Design Notes
When we ordered the gblock from IDT, we requested that it is 5' end phosphorylated to facilitate the blunt end ligation into the pJET backbone vector which is then transformed into E coli. for DNA cloning.
Source
HBV, Hepatitis B Virus
References
https://www.cdc.gov/hepatitis/hbv/index.htm
Kim, Bo Hyun, and W. Ray Kim. "Epidemiology of hepatitis B virus infection in the United States." Clinical liver disease 12.1 (2018): 1.
Kim, W Ray. “Epidemiology of hepatitis B in the United States.” Hepatology (Baltimore, Md.) vol. 49,5 Suppl (2009): S28-34. doi:10.1002/hep.22975
https://www.ncbi.nlm.nih.gov/nuccore/NC_003977.2?report=fasta&from=1816&to=2454